Western Blot, Positive WB detected in: HEK293 whole cell lysate (30ug), MCF-7 whole cell lysate (30ug), SH-SY5Y whole cell lysate (30ug), A375 whole cell lysate (30ug), A549 whole cell lysate (30ug), Jurkat whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes: USP11 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 110 kDa, Observed band size: 110 kDa, Exposure time:2min
Overlay Peak curve showing SH-SY5Y cells stained with RAC08869 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.