Generate High-Specificity Monoclonal Antibodies Faster
Selected Citations for Biomatik’s Custom Antibody Services
Biomatik’s single B cell monoclonal antibody production service provides a powerful alternative to traditional hybridoma methods. We isolate and sequence antigen-specific B cells from immunized animals to generate high-affinity, sequence-defined monoclonal antibodies. This approach captures natural heavy-light chain pairings, and is ideal for difficult targets and projects where timeline and reproducibility are critical.
We can also produce the target antigen peptide or protein for your project through our custom peptide synthesis and recombinant protein production services, offering you a seamless, full-service solution from antigen generation to antibody delivery.
Why Choose Biomatik for Your Monoclonal Antibody Production?
Scientific & Customer Trust: View our selected peer-reviewed citations in Nature, Gastroenterology, and Immunity, alongside our consistent 5-star Google reviews.
Biomatik has over 20 years of experience supporting life sciences research, delivering reliable, flexible custom antibody services to scientists worldwide. Our single B cell monoclonal antibody production service provides:
- Single B cell isolation and cloning, and delivery of 2-5 mg of monoclonal antibody from the top clone
- Sequence data and plasmids of two antibodies from top two positive clones
- Cell supernatant from top 10 positive clones
- Rapid timelines and efficient workflow
- Recombinant-quality monoclonal antibodies
- True natural heavy-light chain pairing
- Optional large-scale recombinant production
- Expert consultation throughout your project
Download full details of our monoclonal antibody production packages for full details on service contents and deliverables.
About Single B Cell Antibody Technology
Single B cell antibody technology is an advanced approach for rapidly isolating and producing high-affinity monoclonal antibodies directly from the B cells of immunized animals. Unlike traditional methods that rely on hybridoma generation (which involves fusing B cells with myeloma cells), single B cell platforms allow researchers to capture native heavy and light chain antibody pairs from individual B cells. This results in fully recombinant monoclonal antibodies with unmatched specificity, consistency, and reproducibility.
The Single B Cell Workflow
The process begins with immunization of an animal – typically mice or New Zealand rabbits – using your antigen of interest. Antigen-specific B cells are then isolated from peripheral blood, spleen, or lymphoid tissues using advanced cell-sorting techniques. These cells are screened and selected based on antigen binding affinity.
The heavy and light chain genes from individual B cells are amplified by PCR, sequenced, and cloned into expression vectors. Finally, the antibody is expressed recombinantly to produce a fully characterized monoclonal antibody.
This method enables:
- Retrieval of natural paired heavy and light chain genes
- Rapid identification of high-affinity binders
- Early sequence availability for future recombinant production or engineering
How Does Single B Cell Technology Compare to Traditional Methods?
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Single B Cell Antibody Technology |
Traditional Hybridoma Method |
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Faster timelines (3–4 months) |
Longer development time (4–6 months) |
|
Captures natural heavy-light chain pairing |
Random pairing during fusion |
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High success rate for difficult targets |
Lower success with weak antigens |
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Fully recombinant antibody output |
Antibodies produced from hybridomas |
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Less screening effort required |
Time-intensive screening needed |
|
Scalable recombinant production from sequence |
Additional cloning steps required for recombinant production |
Single B cell antibody technology is ideal when you need:
- Antibodies against challenging or weakly immunogenic targets
- Rapid turnaround for high-value targets
- Sequence-verified monoclonal antibodies for long-term supply or therapeutic research
- Fully recombinant antibodies for research use
Get Started With Your Project
- Contact our team with your antigen details
- Receive a customized quote and project plan
- Approve and initiate production