Western Blot, Positive WB detected in: K562 whole cell lysate (30ug), JURKAT whole cell lysate (30ug),MCF-7 whole cell lysate (30ug),Hela whole cell lysate (30ug),COLO-205 whole cell lysate (30ug),A549 whole cell lysate (20ug),HepG2 whole cell lysate (30ug), All lanes: TR antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 71 kDa, Observed band size: 57 kDa, Exposure time:2min
Immunofluorescence staining of HepG2 cell with RAC08237 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing Hela cells stained with RAC08237 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.