Western Blot. Positive WB detected in:A431 whole cell lysate (20µg), SH-SY5Y whole cell lysate (20µg), U251 whole cell lysate (20µg), 293T whole cell lysate (20µg), HepG2 whole cell lysate (20µg), K562 whole cell lysate (20µg), MCF7 whole cell lysate (20µg), NIH/3T3 whole cell lysate (20µg). All lanes: TUBB antibody at 1:1000. Secondary. Goat polyclonal to human IgG at 1/40000 dilution. Predicted band size:49 kDa. Observed band size:55 kDa. Exposure time:5s
IHC image diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.