Ultra Sensitive IHC

TBL1XR1 Recombinant Monoclonal Antibody

BiomatikSKU: RAC09114-100ul

Size: 100ul
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Description

Technical Documents

Images

Western Blot, Positive WB detected in: Hela whole cell lysate (30ug), A549 whole cell lysate (30ug), K562 whole cell lysate (30ug), A2780 whole cell lysate (30ug), Jurkat whole cell lysate (30ug), A431 whole cell lysate (30ug), THP-1 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), All lanes:TBL1XR1 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 56 kDa, Observed band size: 56 kDa, Exposure time:2min
IHC image of RAC09114 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing Hela cells stained with RAC09114 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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