Western Blot, Positive WB detected in: A549 whole cell lysatee, All lanes: SYP antibody at 1:500, Secondary, Goat polyclonal to mouse IgG at 1/50000 dilution, Predicted band size: 34 kDa, Observed band size: 34 kDa
IHC image of RAC07980 diluted at 1:200 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela cell with RAC07980 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).