Western Blot, Positive WB detected in: THP-1 whole cell lysate (20ug), A549 whole cell lysate (20ug),K-562 whole cell lysate (20ug),Hela whole cell lysate (20ug),NIH/3T3 whole cell lysate (20ug),JURKAT whole cell lysate (20ug), Mouse liver tissue lysate (20ug), All lanes: STAT5A/B antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 90 kDa, Observed band size: 102 kDa, Exposure time:40s
IHC image of RAC08222 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.