The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibody; Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-SEMA4D recombinant antibody at 2 ug/ml can bind Mouse Sema4d, the EC50 is 5.829-16.86 ng/ml.
The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody; Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D at 2 ug/ml can bind Anti- SEMA4D recombinant antibody, the EC50 is 60.38-152.6 ng/ml.
Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.