Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibody.; Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Sema4d at 2 µg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 98.14-174.8 ng/mL.
The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody.; , Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D at 2 µg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 1.082-4.805 ng/mL.