RPSA Recombinant Monoclonal Antibody, Cat#RAC08250
Western Blot, Positive WB detected in: PC-3 whole cell lysate (30ug), U-251MG whole cell lysate (30ug), K562 whole cell lysate (30ug), HeLa whole cell lysate (30ug), A549 whole cell lysate (30ug), SW620 whole cell lysate (30ug), HepG2 whole cell lysate (30ug), HEK293 whole cell lysate (30ug), NIH/3T3 whole cell lysate (30ug), All lanes: RPSA antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 33 kDa, Observed band size: 42 kDa, Exposure time: 1min
Immunofluorescence staining of HepG2 cell with RAC08250 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing MCF-7 cells stained with RAC08250 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
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RPSA Recombinant Monoclonal Antibody, Cat#RAC08250 Western Blot, Positive WB detected in: PC-3 whole cell lysate (30ug), U-251MG whole cell lysate (30ug), K562 whole cell lysate (30ug), HeLa whole cell lysate (30ug), A549 whole cell lysate (30ug), SW620 whole cell lysate (30ug), HepG2 whole cell lysate (30ug), HEK293 whole cell lysate (30ug), NIH/3T3 whole cell lysate (30ug), All lanes: RPSA antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 33 kDa, Observed band size: 42 kDa, Exposure time: 1min Immunofluorescence staining of HepG2 cell with RAC08250 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay Peak curve showing MCF-7 cells stained with RAC08250 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

RPSA Recombinant Monoclonal Antibody

BiomatikSKU: RAC08250-100ul
Size: 100ul
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Western Blot, Positive WB detected in: PC-3 whole cell lysate (30ug), U-251MG whole cell lysate (30ug), K562 whole cell lysate (30ug), HeLa whole cell lysate (30ug), A549 whole cell lysate (30ug), SW620 whole cell lysate (30ug), HepG2 whole cell lysate (30ug), HEK293 whole cell lysate (30ug), NIH/3T3 whole cell lysate (30ug), All lanes: RPSA antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 33 kDa, Observed band size: 42 kDa, Exposure time: 1min
Immunofluorescence staining of HepG2 cell with RAC08250 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing MCF-7 cells stained with RAC08250 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

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