RAB8A Recombinant Monoclonal Antibody, Cat#RAC08496
Western Blot, Positive WB detected in: Hela whole cell lysate (30ug), PC-3 whole cell lysate (30ug), Raji whole cell lysate (30ug), MCF-7 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes: Rab8A antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 24 kDa, Observed band size: 24 kDa, Exposure time:2min
Immunofluorescence staining of MCF-7 cell with RAC08496 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing Hela cells stained with RAC08496 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
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RAB8A Recombinant Monoclonal Antibody, Cat#RAC08496 Western Blot, Positive WB detected in: Hela whole cell lysate (30ug), PC-3 whole cell lysate (30ug), Raji whole cell lysate (30ug), MCF-7 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes: Rab8A antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 24 kDa, Observed band size: 24 kDa, Exposure time:2min Immunofluorescence staining of MCF-7 cell with RAC08496 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay Peak curve showing Hela cells stained with RAC08496 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

RAB8A Recombinant Monoclonal Antibody

BiomatikSKU: RAC08496-100ul
Size: 100ul
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Western Blot, Positive WB detected in: Hela whole cell lysate (30ug), PC-3 whole cell lysate (30ug), Raji whole cell lysate (30ug), MCF-7 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes: Rab8A antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 24 kDa, Observed band size: 24 kDa, Exposure time:2min
Immunofluorescence staining of MCF-7 cell with RAC08496 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing Hela cells stained with RAC08496 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

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We were very pleased with Biomatik’s performance on a custom recombinant protein project earlier this summer. Communication throughout the project was excellent, technical questions were addressed promptly, and the supporting analytical and QC data were clear and comprehensive. The recombinant protein was produced to the requested specifications and delivered on schedule. Based on this experience, we have chosen Biomatik for an additional custom protein production project and would be pleased to work with them again.

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Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

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We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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Sarah Wootton

The peptide product is of exceptional quality and is highly recommended for its efficacy.

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I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

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I have bought peptides from Biomatic several times and the quality is the best!!!

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