PTK2B Recombinant Monoclonal Antibody, Cat#RAC09051
Western Blot, Positive WB detected in: Jurkat whole cell lysate (30ug), A431 whole cell lysate (30ug), THP-1 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes:PTK2B antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 116 kDa, Observed band size: 116 kDa, Exposure time:1min
Immunofluorescence staining of A549 cell with RAC09051 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing jurkat cells stained with RAC09051 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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PTK2B Recombinant Monoclonal Antibody, Cat#RAC09051 Western Blot, Positive WB detected in: Jurkat whole cell lysate (30ug), A431 whole cell lysate (30ug), THP-1 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes:PTK2B antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 116 kDa, Observed band size: 116 kDa, Exposure time:1min Immunofluorescence staining of A549 cell with RAC09051 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Overlay Peak curve showing jurkat cells stained with RAC09051 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.

PTK2B Recombinant Monoclonal Antibody

BiomatikSKU: RAC09051-100ul
Size: 100ul
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Western Blot, Positive WB detected in: Jurkat whole cell lysate (30ug), A431 whole cell lysate (30ug), THP-1 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes:PTK2B antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 116 kDa, Observed band size: 116 kDa, Exposure time:1min
Immunofluorescence staining of A549 cell with RAC09051 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Overlay Peak curve showing jurkat cells stained with RAC09051 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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We were very pleased with Biomatik’s performance on a custom recombinant protein project earlier this summer. Communication throughout the project was excellent, technical questions were addressed promptly, and the supporting analytical and QC data were clear and comprehensive. The recombinant protein was produced to the requested specifications and delivered on schedule. Based on this experience, we have chosen Biomatik for an additional custom protein production project and would be pleased to work with them again.

Thomas Hatch

We have been ordering several proteins for our research. Biomatik always delivered very good products, and the service was very reliable. Their customer service has always been very responsive and professional. We are very satisfied with this company.

Davide Z

A reliable antibody and peptide synthesis company. We’ve been using Biomtik for polyconal antibodies in our research. Turnaround times are consistent, and communication is always clear and professional.

Hsing Yi Cho

We’ve been using Biomtik for peptide synthesis in our research, and they have proven to be extremely reliable. Turnaround times are consistent, product quality is excellent, and communication is always clear and professional. Highly recommend their services to any lab looking for dependable peptide synthesis.

Tarring Lu

Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

Andrei Baluna

We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

Eduardo Peña

We have ordered peptides from Biomatik several times. Their quality is excellent and they work with you every step of the way to ensure a quality product.

A G

Estoy muy satisfecha con los servicios de síntesis de péptidos de Biomatik. Para mí como investigadora, Biomatik cumplió todas mis expectativas técnicas para realizar mi trabajo experimental. Toda su atención es buena!

Cristina Cevallos

We have purchased peptides from Biomatik for a few years now. Their ordering process was smooth, customer service was responsive and helpful, and the peptides arrived on time and of great quality.

Jeella Acedo

Great service. I'm a returning customer. The product quality and timeline are both outstanding.

Yue Home Liu

We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

Sarah Wootton

The peptide product is of exceptional quality and is highly recommended for its efficacy.

Haoyang Xin

I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

Roi Asor

I have bought peptides from Biomatic several times and the quality is the best!!!

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