Western Blot; Positive WB detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue; All lanes: PTGS1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 69, 65, 62, 57, 72, 73 kDa; Observed band size: 72 kDa
Overlay histogram showing Hela cells stained with RAC07261 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.