Rat Lactate Dehydrogenase C, Active Protein, Cat#RPU60639
SDS-PAGE
A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD( ) that is essential for the continuity of glycolysis. Inhibiting LDH activity has an anti-proliferative effect on cancer cells. It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target. LDH enzymes have three homologous subunits LDHA, LDHB and LDHC. The activity of recombinant rat LDHC was measured by its ability to reduce pyruvate to lactate. The reaction was performed in 25 mM Tris, 100 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of rrLDHC (diluted by assay buffer) to 50 uL of substrate mixture 1.6 mM beta-NADH and 4 mM sodium pyruvate. The final well serves as a negative control with no rrLDHC, replaced with 50 μl assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat LDHC is > 880 pmol/min/ug.
Roll over image to zoom in Click on image to zoom
Rat Lactate Dehydrogenase C, Active Protein, Cat#RPU60639 SDS-PAGE A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD( ) that is essential for the continuity of glycolysis. Inhibiting LDH activity has an anti-proliferative effect on cancer cells. It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target. LDH enzymes have three homologous subunits LDHA, LDHB and LDHC. The activity of recombinant rat LDHC was measured by its ability to reduce pyruvate to lactate. The reaction was performed in 25 mM Tris, 100 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of rrLDHC (diluted by assay buffer) to 50 uL of substrate mixture 1.6 mM beta-NADH and 4 mM sodium pyruvate. The final well serves as a negative control with no rrLDHC, replaced with 50 μl assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat LDHC is > 880 pmol/min/ug.

Rat Lactate Dehydrogenase C, Active Protein

BiomatikSKU: RPU60639-50ug
Size: 50ug
Request Information
!

This item is non-returnable unless there is a quality issue. See Returns Policy
Product Details

Technical Docs

Images

Description

Technical Documents

PDF icon Safety Data Sheet

Images

SDS-PAGE
A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD( ) that is essential for the continuity of glycolysis. Inhibiting LDH activity has an anti-proliferative effect on cancer cells. It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target. LDH enzymes have three homologous subunits LDHA, LDHB and LDHC. The activity of recombinant rat LDHC was measured by its ability to reduce pyruvate to lactate. The reaction was performed in 25 mM Tris, 100 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of rrLDHC (diluted by assay buffer) to 50 uL of substrate mixture 1.6 mM beta-NADH and 4 mM sodium pyruvate. The final well serves as a negative control with no rrLDHC, replaced with 50 μl assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat LDHC is > 880 pmol/min/ug.

What our customers say

Custom Protein Expression Services

“We had concerns about hydrophobic proteins with poor solubility. Biomatik started from codon optimization and gene synthesis, and the resulting proteins had significantly improved solubility. In our ELISA assays, they showed high specificity and low background. Excellent quality, fast turnaround, and helpful support—highly recommended.”

— Dr. Jia, Centers for Disease Control and Prevention, Atlanta, GA, USA

Custom Antibody Production Services

“I work on molecular farming, producing recombinant protein in plant suspension cells. I'm completely satisfied after almost 5 years of ordering antibodies at Biomatik. Perfect reproducibility batch after batch, and I hope to continue like this!”

— Dr. Eduardo Peña, Ergo Bioscience Inc., Wilmington, DE, USA

Custom Antibody Production Services

“We are very satisfied with the peptide antibodies you produced. They work exceptionally well for both WB and immunostaining—strong and specific signals. Your pricing, service, and timelines are excellent. I will definitely continue using your service.”

— Dr. Han, Senior Research Associate, Department of Biology, McGill University, Montreal, QC, Canada

Custom Gene Synthesis Services

“Different groups here have found your service excellent. That’s why another group also decided to go with Biomatik—it came highly recommended.”

— Dr. Heilbronn, College of Life Sciences, University of Dundee, U.K.

Custom Antibody Production Services

“We received the antibody and it works very well! I’m extremely happy with it. We’ll definitely contact you for future antibody projects.”

— Dr. Keeney, Jim Sikela/Diego Restrepo Labs, University of Colorado Anschutz Medical Campus, Aurora, CO, USA

Custom Protein Expression Services

"I'm very happy with Biomatik, because we have had good results with our protein expression. The service is fast and high quality compared with other companies.”

— Dr. Ricardo Godoy, Universidad de La Frontera, Chile

Custom Peptide Synthesis Services

“I’ve ordered many peptides from Biomatik over the past two years. Every experience has been smooth—thanks to their great customer service and unbeatable prices. I’ll continue choosing Biomatik for all my peptide orders.”

— Dr. Tam, Optometry, University of California Berkeley, CA, USA

Custom Protein Expression Services

“I’ve used several companies for peptide synthesis, and Biomatik has been the best. Their prices are competitive—half the cost of some others—and the customer service is top-notch. Ordering is quick and pain-free.”

— Dr. Webb, Department of Cell and Tissue Biology, University of California San Francisco, CA, USA

Custom Antibody Production Servicdes

“Biomatik has generated several primary antibodies for me over the past 8 years. All performed beautifully in WB and IH/IF. I’m extremely pleased with their outstanding service, work ethics, and professionalism. Production was always on time and delivered as quoted. I will definitely return for future antibody needs.”

— Hassane Amlal, Ph.D., Professor, University of Cincinnati, OH, USA

Custom Antibody Production Services

“We ordered polyclonal antibody production using synthetic peptides and received everything on schedule. The antibodies produced strong, specific signals and integrated seamlessly into our experimental protocols. An excellent experience.”

— Professor O'Tousa, Department of Biological Sciences, University of Notre Dame, IN, USA

Let customers speak for us

from 222 reviews

We were very pleased with Biomatik’s performance on a custom recombinant protein project earlier this summer. Communication throughout the project was excellent, technical questions were addressed promptly, and the supporting analytical and QC data were clear and comprehensive. The recombinant protein was produced to the requested specifications and delivered on schedule. Based on this experience, we have chosen Biomatik for an additional custom protein production project and would be pleased to work with them again.

Thomas Hatch

We have been ordering several proteins for our research. Biomatik always delivered very good products, and the service was very reliable. Their customer service has always been very responsive and professional. We are very satisfied with this company.

Davide Z

A reliable antibody and peptide synthesis company. We’ve been using Biomtik for polyconal antibodies in our research. Turnaround times are consistent, and communication is always clear and professional.

Hsing Yi Cho

We’ve been using Biomtik for peptide synthesis in our research, and they have proven to be extremely reliable. Turnaround times are consistent, product quality is excellent, and communication is always clear and professional. Highly recommend their services to any lab looking for dependable peptide synthesis.

Tarring Lu

Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

Andrei Baluna

We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

Eduardo Peña

We have ordered peptides from Biomatik several times. Their quality is excellent and they work with you every step of the way to ensure a quality product.

A G

Estoy muy satisfecha con los servicios de síntesis de péptidos de Biomatik. Para mí como investigadora, Biomatik cumplió todas mis expectativas técnicas para realizar mi trabajo experimental. Toda su atención es buena!

Cristina Cevallos

We have purchased peptides from Biomatik for a few years now. Their ordering process was smooth, customer service was responsive and helpful, and the peptides arrived on time and of great quality.

Jeella Acedo

Great service. I'm a returning customer. The product quality and timeline are both outstanding.

Yue Home Liu

We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

Sarah Wootton

The peptide product is of exceptional quality and is highly recommended for its efficacy.

Haoyang Xin

I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

Roi Asor

I have bought peptides from Biomatic several times and the quality is the best!!!

Kyung Won Park

Recently viewed