Rat Fibroblast Growth Factor 21, Active Protein, Cat#RPU60297
SDS-PAGE
Fibroblast growth factor 21 (FGF21) is a protein that in mammals is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family which possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF21 using Balb/c 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of FGF21 diluted with serum-free standard DMEM. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Balb/c 3T3 cells after incubation with FGF21 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8(Cell Counting Kit-8) assay after incubation with recombinant FGF21 for 48h. It was obvious that FGF21 significantly increased cell viability of 3T3 cells. The ED50 of recombinant rat FGF21 is 0.23 ng/ml.
The dose-effect curve of FGF21 on Balb/c 3T3 cells.
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Rat Fibroblast Growth Factor 21, Active Protein, Cat#RPU60297 SDS-PAGE Fibroblast growth factor 21 (FGF21) is a protein that in mammals is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family which possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF21 using Balb/c 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of FGF21 diluted with serum-free standard DMEM. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Balb/c 3T3 cells after incubation with FGF21 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8(Cell Counting Kit-8) assay after incubation with recombinant FGF21 for 48h. It was obvious that FGF21 significantly increased cell viability of 3T3 cells. The ED50 of recombinant rat FGF21 is 0.23 ng/ml. The dose-effect curve of FGF21 on Balb/c 3T3 cells.

Rat Fibroblast Growth Factor 21, Active Protein

BiomatikSKU: RPU60297-50ug
Size: 50ug
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SDS-PAGE
Fibroblast growth factor 21 (FGF21) is a protein that in mammals is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family which possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF21 using Balb/c 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of FGF21 diluted with serum-free standard DMEM. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Balb/c 3T3 cells after incubation with FGF21 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8(Cell Counting Kit-8) assay after incubation with recombinant FGF21 for 48h. It was obvious that FGF21 significantly increased cell viability of 3T3 cells. The ED50 of recombinant rat FGF21 is 0.23 ng/ml.
The dose-effect curve of FGF21 on Balb/c 3T3 cells.

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