Rat Cluster Of Differentiation 200, Active Protein, Cat#RPU60753
SDS-PAGE
Cluster Of Differentiation 200 (CD200), also known as OX-2 or MOX1/MOX2, is a membrane glycoprotein belonging to the immunoglobulin superfamily. This protein is widely expressed in various cell types, including neurons, endothelial cells, certain immune cells (such as B cells, dendritic cells, T cell subsets), and various tumor cells. CD200 is a multifaceted protein with important roles in immune regulation, neuronal function, and disease pathogenesis. Besides, Cluster Of Differentiation 8a (CD8a) has been identified as an interactor of CD200, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD200 and recombinant human CD8a. Briefly, CD200 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD8a-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD200 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CD200 and recombinant human CD8a was shown, the EC50 for this effect is 0.09 ug/mL.
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Rat Cluster Of Differentiation 200, Active Protein, Cat#RPU60753 SDS-PAGE Cluster Of Differentiation 200 (CD200), also known as OX-2 or MOX1/MOX2, is a membrane glycoprotein belonging to the immunoglobulin superfamily. This protein is widely expressed in various cell types, including neurons, endothelial cells, certain immune cells (such as B cells, dendritic cells, T cell subsets), and various tumor cells. CD200 is a multifaceted protein with important roles in immune regulation, neuronal function, and disease pathogenesis. Besides, Cluster Of Differentiation 8a (CD8a) has been identified as an interactor of CD200, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD200 and recombinant human CD8a. Briefly, CD200 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD8a-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD200 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CD200 and recombinant human CD8a was shown, the EC50 for this effect is 0.09 ug/mL.

Rat Cluster Of Differentiation 200, Active Protein

BiomatikSKU: RPU60753-50ug
Size: 50ug
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SDS-PAGE
Cluster Of Differentiation 200 (CD200), also known as OX-2 or MOX1/MOX2, is a membrane glycoprotein belonging to the immunoglobulin superfamily. This protein is widely expressed in various cell types, including neurons, endothelial cells, certain immune cells (such as B cells, dendritic cells, T cell subsets), and various tumor cells. CD200 is a multifaceted protein with important roles in immune regulation, neuronal function, and disease pathogenesis. Besides, Cluster Of Differentiation 8a (CD8a) has been identified as an interactor of CD200, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD200 and recombinant human CD8a. Briefly, CD200 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD8a-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD200 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CD200 and recombinant human CD8a was shown, the EC50 for this effect is 0.09 ug/mL.

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