Mouse Tumor Necrosis Factor Alpha (TNFa), Active Protein

BiomatikSKU: RPU54793-50ug

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TNF-a, being an endogenous pyrogen, is able to induce fever, necrosis, inflammation and to inhibit tumorigenesis. As reported, TNF-a could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA .<br/>Cell necrosis after incubation with TNF-a (10ng/mL) for 72h was shown in Figure 1.
The production of IL-1β after incubation with TNF-a (10ng/mL) for 8h is shown in Table 1.

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