Mouse Tumor Necrosis Factor Alpha (TNFa), Active Protein

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TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β in cell supernatant was detected by ELISA. Cell apoptosis of A549 after incubation of 48h was shown in Figure 1.

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