Mouse Osteopontin, Active Protein, Cat#RPU60094
SDS-PAGE
Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappaB pathways in MCF-7 breast cancer cell line. To test the bioactivity of recombinant mouse OPN, MCF-7 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of recombinant mouse OPN for 48h and MCP-1 levels in the cell supernatant were determined by ELISA (SEA087Hu). MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the EC50 was 398 ug/ml.
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Mouse Osteopontin, Active Protein, Cat#RPU60094 SDS-PAGE Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappaB pathways in MCF-7 breast cancer cell line. To test the bioactivity of recombinant mouse OPN, MCF-7 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of recombinant mouse OPN for 48h and MCP-1 levels in the cell supernatant were determined by ELISA (SEA087Hu). MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the EC50 was 398 ug/ml.

Mouse Osteopontin, Active Protein

BiomatikSKU: RPU60094-50ug
Size: 50ug
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SDS-PAGE
Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappaB pathways in MCF-7 breast cancer cell line. To test the bioactivity of recombinant mouse OPN, MCF-7 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of recombinant mouse OPN for 48h and MCP-1 levels in the cell supernatant were determined by ELISA (SEA087Hu). MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the EC50 was 398 ug/ml.

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