Mouse Macrophage Inflammatory Protein 1 Alpha, Active Protein, Cat#RPU60400
SDS-PAGE
MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers and various concentrations of MIP-1a was added in lower chamber with a polycarbonate filter (8 um pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted using Image J. The migrated THP1 cells in low chamber at low magnification (×100) was shown. The number of migrated THP-1 cells. The optimum chemotaxis of MIP-1a occurs at 1 ug/ml.
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Mouse Macrophage Inflammatory Protein 1 Alpha, Active Protein, Cat#RPU60400 SDS-PAGE MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers and various concentrations of MIP-1a was added in lower chamber with a polycarbonate filter (8 um pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted using Image J. The migrated THP1 cells in low chamber at low magnification (×100) was shown. The number of migrated THP-1 cells. The optimum chemotaxis of MIP-1a occurs at 1 ug/ml.

Mouse Macrophage Inflammatory Protein 1 Alpha, Active Protein

BiomatikSKU: RPU60400-50ug
Size: 50ug
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SDS-PAGE
MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers and various concentrations of MIP-1a was added in lower chamber with a polycarbonate filter (8 um pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted using Image J. The migrated THP1 cells in low chamber at low magnification (×100) was shown. The number of migrated THP-1 cells. The optimum chemotaxis of MIP-1a occurs at 1 ug/ml.

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