Mouse Interleukin 3, Active Protein, Cat#RPU60395
SDS-PAGE
Interleukin 3 is an interleukin, a type of biological signal that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin 3 receptor IL-3 synergizes with other cytokines to stimulate the growth of immature progenitor cells of all lineages, and is therefore a multi-lineage colony-stimulating factor (CSF). It prevents cell death and promotes the survival of macrophages, mast cells, and megakaryocytes. To test the effect of IL-3 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant mouse IL-3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 °C. Proliferation of TF-1 cells after incubation with IL-3 for72h observed by inverted microscope. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant IL-3 for 72h. It was obvious that IL-3 significantly increased cell viability of TF-1 cells. The ED50 is 3.79 ng/ml.
The dose-effect curve of IL-3 on TF-1 cells
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Mouse Interleukin 3, Active Protein, Cat#RPU60395 SDS-PAGE Interleukin 3 is an interleukin, a type of biological signal that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin 3 receptor IL-3 synergizes with other cytokines to stimulate the growth of immature progenitor cells of all lineages, and is therefore a multi-lineage colony-stimulating factor (CSF). It prevents cell death and promotes the survival of macrophages, mast cells, and megakaryocytes. To test the effect of IL-3 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant mouse IL-3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 °C. Proliferation of TF-1 cells after incubation with IL-3 for72h observed by inverted microscope. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant IL-3 for 72h. It was obvious that IL-3 significantly increased cell viability of TF-1 cells. The ED50 is 3.79 ng/ml. The dose-effect curve of IL-3 on TF-1 cells

Mouse Interleukin 3, Active Protein

BiomatikSKU: RPU60395-50ug
Size: 50ug
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SDS-PAGE
Interleukin 3 is an interleukin, a type of biological signal that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin 3 receptor IL-3 synergizes with other cytokines to stimulate the growth of immature progenitor cells of all lineages, and is therefore a multi-lineage colony-stimulating factor (CSF). It prevents cell death and promotes the survival of macrophages, mast cells, and megakaryocytes. To test the effect of IL-3 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant mouse IL-3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37 °C. Proliferation of TF-1 cells after incubation with IL-3 for72h observed by inverted microscope. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant IL-3 for 72h. It was obvious that IL-3 significantly increased cell viability of TF-1 cells. The ED50 is 3.79 ng/ml.
The dose-effect curve of IL-3 on TF-1 cells

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