Mouse Histidine Rich Glycoprotein, Active Protein, Cat#RPU60355
SDS-PAGE
Human histidine-rich glycoprotein (HRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. As HRG has the function of cell adhesion, we measure the activity of recombinant mouse HRG by the ability of the immobilized protein to support the adhesion of con-A activated MOLT4 human acute lymphoblastic leukemia cells. When 1 x 105 cells/well with 7.5 ug/ml conA are added to recombinat mouse HRG coated plates (different concentrations with 100 uL/well), cells will adhere after 2 hour incubation at 37 °C. The adhesion of MOLT-4 after 2 hour incubation at 37 °C observed by inverted microscope was shown. The result of cell adhesion, the EC50 was 0.36 ug/ml.
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Mouse Histidine Rich Glycoprotein, Active Protein, Cat#RPU60355 SDS-PAGE Human histidine-rich glycoprotein (HRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. As HRG has the function of cell adhesion, we measure the activity of recombinant mouse HRG by the ability of the immobilized protein to support the adhesion of con-A activated MOLT4 human acute lymphoblastic leukemia cells. When 1 x 105 cells/well with 7.5 ug/ml conA are added to recombinat mouse HRG coated plates (different concentrations with 100 uL/well), cells will adhere after 2 hour incubation at 37 °C. The adhesion of MOLT-4 after 2 hour incubation at 37 °C observed by inverted microscope was shown. The result of cell adhesion, the EC50 was 0.36 ug/ml.

Mouse Histidine Rich Glycoprotein, Active Protein

BiomatikSKU: RPU60355-50ug
Size: 50ug
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SDS-PAGE
Human histidine-rich glycoprotein (HRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. As HRG has the function of cell adhesion, we measure the activity of recombinant mouse HRG by the ability of the immobilized protein to support the adhesion of con-A activated MOLT4 human acute lymphoblastic leukemia cells. When 1 x 105 cells/well with 7.5 ug/ml conA are added to recombinat mouse HRG coated plates (different concentrations with 100 uL/well), cells will adhere after 2 hour incubation at 37 °C. The adhesion of MOLT-4 after 2 hour incubation at 37 °C observed by inverted microscope was shown. The result of cell adhesion, the EC50 was 0.36 ug/ml.

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