Mouse Galectin 2, Active Protein, Cat#RPU60337
SDS-PAGE
Galectin 2 (GAL2) belongs to the proto type group and consists of two homologous carbohydrate recognition domains (CRDs) resulting in multiple sugar binding sites. The expression of gal-2 has been shown to be involved in processes of angiogenesis and inflammation but was not analyzed before in preeclamptic (PE) placentas.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant mouse GAL2 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL2 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL2, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown. It was obvious that the minimal effective concentration of GAL2 is 5 μg/ml.
The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.
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Mouse Galectin 2, Active Protein, Cat#RPU60337 SDS-PAGE Galectin 2 (GAL2) belongs to the proto type group and consists of two homologous carbohydrate recognition domains (CRDs) resulting in multiple sugar binding sites. The expression of gal-2 has been shown to be involved in processes of angiogenesis and inflammation but was not analyzed before in preeclamptic (PE) placentas.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant mouse GAL2 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL2 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL2, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown. It was obvious that the minimal effective concentration of GAL2 is 5 μg/ml. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.

Mouse Galectin 2, Active Protein

BiomatikSKU: RPU60337-50ug
Size: 50ug
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SDS-PAGE
Galectin 2 (GAL2) belongs to the proto type group and consists of two homologous carbohydrate recognition domains (CRDs) resulting in multiple sugar binding sites. The expression of gal-2 has been shown to be involved in processes of angiogenesis and inflammation but was not analyzed before in preeclamptic (PE) placentas.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant mouse GAL2 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL2 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL2, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown. It was obvious that the minimal effective concentration of GAL2 is 5 μg/ml.
The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.

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