Mouse Cluster Of Differentiation 28, Active Protein, Cat#RPU60651
SDS-PAGE
The cluster of differentiation (CD) antigen CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. It is a member of the immunoglobulin subfamily, other members of the subfamily include ICOS, CTLA4, PD1, PD1H, TIGIT and BTLA. Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) and CD28 are highly homologous and compete for the same ligands, CTLA4 can also bind with CD28 to form a dimer. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CD28 and recombinant rat CTLA4. Briefly, rmCD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CTLA4-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse CD28 and recombinant rat CTLA4 was shown, the EC50 for this effect is 2.82 ug/mL.
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Mouse Cluster Of Differentiation 28, Active Protein, Cat#RPU60651 SDS-PAGE The cluster of differentiation (CD) antigen CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. It is a member of the immunoglobulin subfamily, other members of the subfamily include ICOS, CTLA4, PD1, PD1H, TIGIT and BTLA. Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) and CD28 are highly homologous and compete for the same ligands, CTLA4 can also bind with CD28 to form a dimer. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CD28 and recombinant rat CTLA4. Briefly, rmCD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CTLA4-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse CD28 and recombinant rat CTLA4 was shown, the EC50 for this effect is 2.82 ug/mL.

Mouse Cluster Of Differentiation 28, Active Protein

BiomatikSKU: RPU60651-50ug
Size: 50ug
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SDS-PAGE
The cluster of differentiation (CD) antigen CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. It is a member of the immunoglobulin subfamily, other members of the subfamily include ICOS, CTLA4, PD1, PD1H, TIGIT and BTLA. Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) and CD28 are highly homologous and compete for the same ligands, CTLA4 can also bind with CD28 to form a dimer. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CD28 and recombinant rat CTLA4. Briefly, rmCD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CTLA4-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse CD28 and recombinant rat CTLA4 was shown, the EC50 for this effect is 2.82 ug/mL.

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