Mouse Carboxylesterase 1 (CES1), Active Protein, Cat#RPU56309
Figure . SDS-PAGE
Figure . Western Blot
Figure . Gene Sequencing (Extract)
Carboxylesterase 1 (CES1) also known as liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant mouse CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50mM Tris, pH 7.5 (Assay Buffer), ainitiated by addition 50μL of various concentrations of CES1 (dilute by Assay Buffer) to 50µL of 2mM Substrate 4-NPA (100mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400nm. One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant mouse CES1 is 1648 pmol/min/µg.Specific Activity (pmol/min/µg)= △OD=Adjusted for Substrate BlankF=Conversion Factor(convert from standard curve of 4-Nitrophenol)T= TimeN=Amount of enzymeFigure. The standard curve of 4-Nitrophenol
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Mouse Carboxylesterase 1 (CES1), Active Protein, Cat#RPU56309 Figure . SDS-PAGE Figure . Western Blot Figure . Gene Sequencing (Extract) Carboxylesterase 1 (CES1) also known as liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant mouse CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50mM Tris, pH 7.5 (Assay Buffer), ainitiated by addition 50μL of various concentrations of CES1 (dilute by Assay Buffer) to 50µL of 2mM Substrate 4-NPA (100mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400nm. One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant mouse CES1 is 1648 pmol/min/µg.Specific Activity (pmol/min/µg)= △OD=Adjusted for Substrate BlankF=Conversion Factor(convert from standard curve of 4-Nitrophenol)T= TimeN=Amount of enzymeFigure. The standard curve of 4-Nitrophenol

Mouse Carboxylesterase 1 (CES1), Active Protein

BiomatikSKU: RPU56309-50ug
Size: 50ug
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Figure . SDS-PAGE
Figure . Western Blot
Figure . Gene Sequencing (Extract)
Carboxylesterase 1 (CES1) also known as liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant mouse CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50mM Tris, pH 7.5 (Assay Buffer), ainitiated by addition 50μL of various concentrations of CES1 (dilute by Assay Buffer) to 50µL of 2mM Substrate 4-NPA (100mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400nm. One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant mouse CES1 is 1648 pmol/min/µg.Specific Activity (pmol/min/µg)= △OD=Adjusted for Substrate BlankF=Conversion Factor(convert from standard curve of 4-Nitrophenol)T= TimeN=Amount of enzymeFigure. The standard curve of 4-Nitrophenol

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