Human Toll Like Receptor 4, Active Protein, Cat#RPU60488
SDS-PAGE
TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLRs plays a fundamental role in pathogen recognition and activation of innate immunity. Besides, TLR4 is the receptor for LPS and plays a critical role in innate immunity. Stimulation of TLR4 activates proinflammatory pathways and induces cytokine expression in a variety of cell types. Inflammatory pathways are activated in tissues of obese animals and humans and play an important role in obesity-associated insulin resistance. It is reported that RETN has been identified as an interactor of TLR4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RETN and recombinant human TLR4. Briefly, RETN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TLR4-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-RETN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of TLR4 and RETN was shown. When recombinant human TLR4 is lmmobilized at 2 ug/mL (100 uL/well), the concentration of rhRETN that produces 50% optimal binding response is found to be approximately 6.25 ug/mL.
Roll over image to zoom in Click on image to zoom
Human Toll Like Receptor 4, Active Protein, Cat#RPU60488 SDS-PAGE TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLRs plays a fundamental role in pathogen recognition and activation of innate immunity. Besides, TLR4 is the receptor for LPS and plays a critical role in innate immunity. Stimulation of TLR4 activates proinflammatory pathways and induces cytokine expression in a variety of cell types. Inflammatory pathways are activated in tissues of obese animals and humans and play an important role in obesity-associated insulin resistance. It is reported that RETN has been identified as an interactor of TLR4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RETN and recombinant human TLR4. Briefly, RETN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TLR4-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-RETN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of TLR4 and RETN was shown. When recombinant human TLR4 is lmmobilized at 2 ug/mL (100 uL/well), the concentration of rhRETN that produces 50% optimal binding response is found to be approximately 6.25 ug/mL.

Human Toll Like Receptor 4, Active Protein

BiomatikSKU: RPU60488-50ug
Size: 50ug
Request Information
!

This item is non-returnable unless there is a quality issue. See Returns Policy
Product Details

Technical Docs

Images

Description

Technical Documents

PDF icon Safety Data Sheet

Images

SDS-PAGE
TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. TLRs plays a fundamental role in pathogen recognition and activation of innate immunity. Besides, TLR4 is the receptor for LPS and plays a critical role in innate immunity. Stimulation of TLR4 activates proinflammatory pathways and induces cytokine expression in a variety of cell types. Inflammatory pathways are activated in tissues of obese animals and humans and play an important role in obesity-associated insulin resistance. It is reported that RETN has been identified as an interactor of TLR4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RETN and recombinant human TLR4. Briefly, RETN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TLR4-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-RETN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of TLR4 and RETN was shown. When recombinant human TLR4 is lmmobilized at 2 ug/mL (100 uL/well), the concentration of rhRETN that produces 50% optimal binding response is found to be approximately 6.25 ug/mL.

Let customers speak for us

from 218 reviews

We were very pleased with Biomatik’s performance on a custom recombinant protein project earlier this summer. Communication throughout the project was excellent, technical questions were addressed promptly, and the supporting analytical and QC data were clear and comprehensive. The recombinant protein was produced to the requested specifications and delivered on schedule. Based on this experience, we have chosen Biomatik for an additional custom protein production project and would be pleased to work with them again.

Thomas Hatch

We have been ordering several proteins for our research. Biomatik always delivered very good products, and the service was very reliable. Their customer service has always been very responsive and professional. We are very satisfied with this company.

Davide Z

A reliable antibody and peptide synthesis company. We’ve been using Biomtik for polyconal antibodies in our research. Turnaround times are consistent, and communication is always clear and professional.

Hsing Yi Cho

We’ve been using Biomtik for peptide synthesis in our research, and they have proven to be extremely reliable. Turnaround times are consistent, product quality is excellent, and communication is always clear and professional. Highly recommend their services to any lab looking for dependable peptide synthesis.

Tarring Lu

Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

Andrei Baluna

We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

Eduardo Peña

We have ordered peptides from Biomatik several times. Their quality is excellent and they work with you every step of the way to ensure a quality product.

A G

Estoy muy satisfecha con los servicios de síntesis de péptidos de Biomatik. Para mí como investigadora, Biomatik cumplió todas mis expectativas técnicas para realizar mi trabajo experimental. Toda su atención es buena!

Cristina Cevallos

We have purchased peptides from Biomatik for a few years now. Their ordering process was smooth, customer service was responsive and helpful, and the peptides arrived on time and of great quality.

Jeella Acedo

Great service. I'm a returning customer. The product quality and timeline are both outstanding.

Yue Home Liu

We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

Sarah Wootton

The peptide product is of exceptional quality and is highly recommended for its efficacy.

Haoyang Xin

I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

Roi Asor

I have bought peptides from Biomatic several times and the quality is the best!!!

Kyung Won Park

Recently viewed