Human Thymosin Beta 4, Active Protein, Cat#RPU60437
SDS-PAGE
Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. It Plays an important role in the organization of the cytoskeleton. It is reported that TMSB4X can combinant with PFN1 to inhibit actin polymerization. A binding ELISA assay was conducted to detect the interaction of recombinant human TMSB4X and recombinant human PFN1. Briefly, TMSB4X were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PFN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TMSB4X pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TMSB4X and PFN1 was shown, the EC50 for this effect is 2.98 ng/mL.
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Human Thymosin Beta 4, Active Protein, Cat#RPU60437 SDS-PAGE Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. It Plays an important role in the organization of the cytoskeleton. It is reported that TMSB4X can combinant with PFN1 to inhibit actin polymerization. A binding ELISA assay was conducted to detect the interaction of recombinant human TMSB4X and recombinant human PFN1. Briefly, TMSB4X were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PFN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TMSB4X pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TMSB4X and PFN1 was shown, the EC50 for this effect is 2.98 ng/mL.

Human Thymosin Beta 4, Active Protein

BiomatikSKU: RPU60437-50ug
Size: 50ug
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SDS-PAGE
Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. It Plays an important role in the organization of the cytoskeleton. It is reported that TMSB4X can combinant with PFN1 to inhibit actin polymerization. A binding ELISA assay was conducted to detect the interaction of recombinant human TMSB4X and recombinant human PFN1. Briefly, TMSB4X were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PFN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TMSB4X pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TMSB4X and PFN1 was shown, the EC50 for this effect is 2.98 ng/mL.

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