Human Nerve Growth Factor, Active Protein, Cat#RPU60627
SDS-PAGE
Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. To test the effect of NGF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with various concentrations of recombinant human NGF. After incubated for 72 hours, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of TF-1 cells after incubation with NGF for 72 hours observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human NGF for 72 hours. It was obvious that recombinant human NGF significantly increased cell viability of TF-1 cells, the EC50 was 0.38 ng/ml.
Cell proliferation of TF-1 cells after stimulated with recombinant human NGF.
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Human Nerve Growth Factor, Active Protein, Cat#RPU60627 SDS-PAGE Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. To test the effect of NGF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with various concentrations of recombinant human NGF. After incubated for 72 hours, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of TF-1 cells after incubation with NGF for 72 hours observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human NGF for 72 hours. It was obvious that recombinant human NGF significantly increased cell viability of TF-1 cells, the EC50 was 0.38 ng/ml. Cell proliferation of TF-1 cells after stimulated with recombinant human NGF.

Human Nerve Growth Factor, Active Protein

BiomatikSKU: RPU60627-50ug
Size: 50ug
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SDS-PAGE
Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. To test the effect of NGF on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 20,000 cells/well with various concentrations of recombinant human NGF. After incubated for 72 hours, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of TF-1 cells after incubation with NGF for 72 hours observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human NGF for 72 hours. It was obvious that recombinant human NGF significantly increased cell viability of TF-1 cells, the EC50 was 0.38 ng/ml.
Cell proliferation of TF-1 cells after stimulated with recombinant human NGF.

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