Human Interleukin 5, Active Protein, Cat#RPU60733
SDS-PAGE
Interleukin-5 (IL-5), a secreted glycoprotein, is a member of  the hematopoietic family. Interleukin 5 has been shown to  stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of recombinant human IL-5 at 37 °C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C . Cell proliferation of TF-1 cells after incubation with IL-5 for 72h observed by inverted microscope was shown. The dose-effect curve of recombinant human IL-5. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 35 ng/ml.
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Human Interleukin 5, Active Protein, Cat#RPU60733 SDS-PAGE Interleukin-5 (IL-5), a secreted glycoprotein, is a member of  the hematopoietic family. Interleukin 5 has been shown to  stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of recombinant human IL-5 at 37 °C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C . Cell proliferation of TF-1 cells after incubation with IL-5 for 72h observed by inverted microscope was shown. The dose-effect curve of recombinant human IL-5. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 35 ng/ml.

Human Interleukin 5, Active Protein

BiomatikSKU: RPU60733-50ug
Size: 50ug
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SDS-PAGE
Interleukin-5 (IL-5), a secreted glycoprotein, is a member of the hematopoietic family. Interleukin 5 has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of recombinant human IL-5 at 37 °C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 °C . Cell proliferation of TF-1 cells after incubation with IL-5 for 72h observed by inverted microscope was shown. The dose-effect curve of recombinant human IL-5. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 35 ng/ml.

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