Human Interleukin 3 Receptor Alpha, Active Protein, Cat#RPU60436
SDS-PAGE
Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. Both the alpha and the beta subunits are members of the cytokine receptor superfamily. A binding ELISA assay was conducted to detect the interaction of recombinant human Interleukin 3 Receptor Alpha and recombinant human Interleukin 3. Briefly, IL3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IL3Ra-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL3Ra and IL3 was shown.
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Human Interleukin 3 Receptor Alpha, Active Protein, Cat#RPU60436 SDS-PAGE Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. Both the alpha and the beta subunits are members of the cytokine receptor superfamily. A binding ELISA assay was conducted to detect the interaction of recombinant human Interleukin 3 Receptor Alpha and recombinant human Interleukin 3. Briefly, IL3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IL3Ra-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL3Ra and IL3 was shown.

Human Interleukin 3 Receptor Alpha, Active Protein

BiomatikSKU: RPU60436-50ug
Size: 50ug
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SDS-PAGE
Interleukin 3 receptor alpha (IL3RA), also known as CD123 (Cluster of Differentiation 123), is a subunit of the functional high-affinity human IL-3 receptor which is a heterodimer. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. Both the alpha and the beta subunits are members of the cytokine receptor superfamily. A binding ELISA assay was conducted to detect the interaction of recombinant human Interleukin 3 Receptor Alpha and recombinant human Interleukin 3. Briefly, IL3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IL3Ra-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL3Ra and IL3 was shown.

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