Human Integrin Alpha X, Active Protein, Cat#RPU60414
SDS-PAGE
Integrin Alpha X (CD11c) is a member of the leukointegrin family, it is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta 2. One of the earliest described properties of CD11c is its ability to mediate phagocytosis of iC3b-opsonized particles. Accordingly, CD11c is known as complement receptor 4. CD11c has also been implicated in phagocytosis of latex beads and bacteria in the absence of complement. CD11c binds a diverse array of ligands such as cell adhesion molecules and Interleukin 13 (IL13). Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD11c and recombinant bovine IL13. Briefly, CD11c was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL13-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD11c pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD11c and recombinant bovine IL13 was shown, the EC50 for this effect is 0.28 ug/mL.
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Human Integrin Alpha X, Active Protein, Cat#RPU60414 SDS-PAGE Integrin Alpha X (CD11c) is a member of the leukointegrin family, it is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta 2. One of the earliest described properties of CD11c is its ability to mediate phagocytosis of iC3b-opsonized particles. Accordingly, CD11c is known as complement receptor 4. CD11c has also been implicated in phagocytosis of latex beads and bacteria in the absence of complement. CD11c binds a diverse array of ligands such as cell adhesion molecules and Interleukin 13 (IL13). Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD11c and recombinant bovine IL13. Briefly, CD11c was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL13-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD11c pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD11c and recombinant bovine IL13 was shown, the EC50 for this effect is 0.28 ug/mL.

Human Integrin Alpha X, Active Protein

BiomatikSKU: RPU60414-50ug
Size: 50ug
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SDS-PAGE
Integrin Alpha X (CD11c) is a member of the leukointegrin family, it is an approximately 150 kDa type I transmembrane glycoprotein that heterodimerizes with Integrin beta 2. One of the earliest described properties of CD11c is its ability to mediate phagocytosis of iC3b-opsonized particles. Accordingly, CD11c is known as complement receptor 4. CD11c has also been implicated in phagocytosis of latex beads and bacteria in the absence of complement. CD11c binds a diverse array of ligands such as cell adhesion molecules and Interleukin 13 (IL13). Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD11c and recombinant bovine IL13. Briefly, CD11c was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL13-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CD11c pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD11c and recombinant bovine IL13 was shown, the EC50 for this effect is 0.28 ug/mL.

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