Human Granzyme K, Active Protein, Cat#RPU59983
SDS-PAGE
Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Human granzyme K is synthesized as a precursor (264 residues) with a signal peptide (residues 124), a propeptide (residues 25-26) and a mature chain (residues 27-264). The purified recombinant human Granzyme K consists of residues 27 to 264 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMK (diluted by assay buffer) to 50 uL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme K is >220 pmol/min/ug.
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Human Granzyme K, Active Protein, Cat#RPU59983 SDS-PAGE Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Human granzyme K is synthesized as a precursor (264 residues) with a signal peptide (residues 124), a propeptide (residues 25-26) and a mature chain (residues 27-264). The purified recombinant human Granzyme K consists of residues 27 to 264 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMK (diluted by assay buffer) to 50 uL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme K is >220 pmol/min/ug.

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Human Granzyme K, Active Protein

BiomatikSKU: RPU59983-50ug
Size: 50ug
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SDS-PAGE
Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Human granzyme K is synthesized as a precursor (264 residues) with a signal peptide (residues 124), a propeptide (residues 25-26) and a mature chain (residues 27-264). The purified recombinant human Granzyme K consists of residues 27 to 264 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMK (diluted by assay buffer) to 50 uL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme K is >220 pmol/min/ug.

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We were very pleased with Biomatik’s performance on a custom recombinant protein project earlier this summer. Communication throughout the project was excellent, technical questions were addressed promptly, and the supporting analytical and QC data were clear and comprehensive. The recombinant protein was produced to the requested specifications and delivered on schedule. Based on this experience, we have chosen Biomatik for an additional custom protein production project and would be pleased to work with them again.

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Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

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