Chicken Interferon Alpha, Active Protein, Cat#RPU60480
SDS-PAGE
Interferon-alpha (IFNa), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits. IFN-α has both anti-viral and immunomodulatory activities on target cells. To test the effect of IFNa on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant chicken IFNa diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Apoptosis of A549 cells after incubation with IFNa for 72h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant chicken IFNa for 72h. It was obvious that IFNa significantly decreased cell viability of A549 cells. The ED50 of recombinant chicken IFNa is 5.13 ug/ml.
Inhibition of A549 cells proliferation after stimulated with recombinant chicken IFN-α.
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Chicken Interferon Alpha, Active Protein, Cat#RPU60480 SDS-PAGE Interferon-alpha (IFNa), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits. IFN-α has both anti-viral and immunomodulatory activities on target cells. To test the effect of IFNa on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant chicken IFNa diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Apoptosis of A549 cells after incubation with IFNa for 72h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant chicken IFNa for 72h. It was obvious that IFNa significantly decreased cell viability of A549 cells. The ED50 of recombinant chicken IFNa is 5.13 ug/ml. Inhibition of A549 cells proliferation after stimulated with recombinant chicken IFN-α.

Chicken Interferon Alpha, Active Protein

BiomatikSKU: RPU60480-50ug
Size: 50ug
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SDS-PAGE
Interferon-alpha (IFNa), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits. IFN-α has both anti-viral and immunomodulatory activities on target cells. To test the effect of IFNa on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant chicken IFNa diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. Apoptosis of A549 cells after incubation with IFNa for 72h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant chicken IFNa for 72h. It was obvious that IFNa significantly decreased cell viability of A549 cells. The ED50 of recombinant chicken IFNa is 5.13 ug/ml.
Inhibition of A549 cells proliferation after stimulated with recombinant chicken IFN-α.

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