Western Blot; Positive WB detected in: Raji whole cell lysate, Hela whole cell lysate, HL60 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate; All lanes: PRMT5 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 73, 72, 54, 68, 67 kDa; Observed band size: 73 kDa
IHC image of RAC07684 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.