Phospho-Stat3 (S727) Recombinant Antibody, Cat#RAC07657
Western Blot; Positive WB detected in A549 whole cell lysate(treated with EGF or not); All lanes Phospho-STAT3 antibody at 1.88ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 98 KDa; Observed band size: 98 KDa
IHC image of RAC07657 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-STAT3 in 293 whole cell lysate treated with Calyculin A; Lane 1: Rabbit control IgG(1ug)instead of RAC07657 in 293 whole cell lysate treated with Calyculin A.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07657(3ug)+ 293 whole cell lysate treated with Calyculin A(1mg); Lane 3: 293 whole cell lysate treated with Calyculin A (20ug)
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Phospho-Stat3 (S727) Recombinant Antibody, Cat#RAC07657 Western Blot; Positive WB detected in A549 whole cell lysate(treated with EGF or not); All lanes Phospho-STAT3 antibody at 1.88ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 98 KDa; Observed band size: 98 KDa IHC image of RAC07657 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating Phospho-STAT3 in 293 whole cell lysate treated with Calyculin A; Lane 1: Rabbit control IgG(1ug)instead of RAC07657 in 293 whole cell lysate treated with Calyculin A.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07657(3ug)+ 293 whole cell lysate treated with Calyculin A(1mg); Lane 3: 293 whole cell lysate treated with Calyculin A (20ug)

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Phospho-Stat3 (S727) Recombinant Antibody

BiomatikSKU: RAC07657-100ul
Size: 100ul
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Western Blot; Positive WB detected in A549 whole cell lysate(treated with EGF or not); All lanes Phospho-STAT3 antibody at 1.88ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 98 KDa; Observed band size: 98 KDa
IHC image of RAC07657 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-STAT3 in 293 whole cell lysate treated with Calyculin A; Lane 1: Rabbit control IgG(1ug)instead of RAC07657 in 293 whole cell lysate treated with Calyculin A.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07657(3ug)+ 293 whole cell lysate treated with Calyculin A(1mg); Lane 3: 293 whole cell lysate treated with Calyculin A (20ug)

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