Phospho-Rps6Ka1 (T359+S363) Recombinant Antibody, Cat#RAC07648
Western Blot; Positive WB detected in HepG2 whole cell lysate(treated with EGF or not); All lanes Phospho-RPS6KA1 antibody at 0.69ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 90 KDa; Observed band size: 90 KDa
Immunofluorescence staining of Hela cells with RAC07648 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-RPS6KA1 in Hela whole cell lysate; Lane 1: Rabbit control IgG(1ug)instead of RAC07648 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07648(3ug)+ Hela whole cell lysate(1mg); Lane 3: Hela whole cell lysate (20ug)
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Phospho-Rps6Ka1 (T359+S363) Recombinant Antibody, Cat#RAC07648 Western Blot; Positive WB detected in HepG2 whole cell lysate(treated with EGF or not); All lanes Phospho-RPS6KA1 antibody at 0.69ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 90 KDa; Observed band size: 90 KDa Immunofluorescence staining of Hela cells with RAC07648 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating Phospho-RPS6KA1 in Hela whole cell lysate; Lane 1: Rabbit control IgG(1ug)instead of RAC07648 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07648(3ug)+ Hela whole cell lysate(1mg); Lane 3: Hela whole cell lysate (20ug)

Phospho-Rps6Ka1 (T359+S363) Recombinant Antibody

BiomatikSKU: RAC07648-100ul
Size: 100ul
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Western Blot; Positive WB detected in HepG2 whole cell lysate(treated with EGF or not); All lanes Phospho-RPS6KA1 antibody at 0.69ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 90 KDa; Observed band size: 90 KDa
Immunofluorescence staining of Hela cells with RAC07648 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-RPS6KA1 in Hela whole cell lysate; Lane 1: Rabbit control IgG(1ug)instead of RAC07648 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07648(3ug)+ Hela whole cell lysate(1mg); Lane 3: Hela whole cell lysate (20ug)

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