Phospho-Polr2A (S2) Recombinant Antibody, Cat#RAC07012
Western Blot; Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysate; All lanes Phospho-POLR2A antibody at 1.02µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 270 KDa; Observed band size: 270 KDa;
IHC image diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07012 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-POLR2A in Hela whole cell lysate; , Lane 1: Rabbit control IgG(1µg)instead in Hela whole cell lysate., For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07012(3µg)+ Hela whole cell lysate(1mg); , Lane 3: Hela whole cell lysate (20µg);
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Phospho-Polr2A (S2) Recombinant Antibody, Cat#RAC07012 Western Blot; Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysate; All lanes Phospho-POLR2A antibody at 1.02µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 270 KDa; Observed band size: 270 KDa; IHC image diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with RAC07012 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating Phospho-POLR2A in Hela whole cell lysate; , Lane 1: Rabbit control IgG(1µg)instead in Hela whole cell lysate., For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07012(3µg)+ Hela whole cell lysate(1mg); , Lane 3: Hela whole cell lysate (20µg);

Ultra Sensitive IHC

Phospho-Polr2A (S2) Recombinant Antibody

BiomatikSKU: RAC07012-100ul
Size: 100ul
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Western Blot; Positive WB detected in Hela whole cell lysate,A549 whole cell lysate,293 whole cell lysate; All lanes Phospho-POLR2A antibody at 1.02µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 270 KDa; Observed band size: 270 KDa;
IHC image diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07012 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-POLR2A in Hela whole cell lysate; , Lane 1: Rabbit control IgG(1µg)instead in Hela whole cell lysate., For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07012(3µg)+ Hela whole cell lysate(1mg); , Lane 3: Hela whole cell lysate (20µg);

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