Phospho-Jak2 (Y1007 + Y1008) Recombinant Antibody, Cat#RAC07615
Western Blot; Positive WB detected in Hela whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not); All lanes Phospho-JAK2 antibody at 0.75ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 120 KDa; Observed band size: 120 KDa
IHC image of RAC07615 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of RAC07615 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-JAK2 in Hela whole cell lysate treated with Pervanadate; Lane 1: Rabbit control IgG(1ug)instead of RAC07615 in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07615(3ug)+ Hela whole cell lysate treated with Pervanadate(1mg); Lane 3: Hela whole cell lysate treated with Pervanadate(20ug)
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Phospho-Jak2 (Y1007 + Y1008) Recombinant Antibody, Cat#RAC07615 Western Blot; Positive WB detected in Hela whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not); All lanes Phospho-JAK2 antibody at 0.75ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 120 KDa; Observed band size: 120 KDa IHC image of RAC07615 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of RAC07615 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating Phospho-JAK2 in Hela whole cell lysate treated with Pervanadate; Lane 1: Rabbit control IgG(1ug)instead of RAC07615 in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07615(3ug)+ Hela whole cell lysate treated with Pervanadate(1mg); Lane 3: Hela whole cell lysate treated with Pervanadate(20ug)

Ultra Sensitive IHC

Phospho-Jak2 (Y1007 + Y1008) Recombinant Antibody

BiomatikSKU: RAC07615-100ul
Size: 100ul
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Western Blot; Positive WB detected in Hela whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not); All lanes Phospho-JAK2 antibody at 0.75ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 120 KDa; Observed band size: 120 KDa
IHC image of RAC07615 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of RAC07615 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-JAK2 in Hela whole cell lysate treated with Pervanadate; Lane 1: Rabbit control IgG(1ug)instead of RAC07615 in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07615(3ug)+ Hela whole cell lysate treated with Pervanadate(1mg); Lane 3: Hela whole cell lysate treated with Pervanadate(20ug)

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