Phospho-Cdk2 (Y15) Recombinant Antibody, Cat#RAC07011
Western Blot; Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not); All lanes:Phospho-CDK2 antibody at 0.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34 KDa; Observed band size: 34 KDa;
Immunocytochemistry analysis diluted at 1:80 and staining in Hela cells(treated with Pervanadate) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-CDK2 in Hela whole cell lysate treated with Pervanadate; Lane 1: Rabbit control IgG(1µg)instead in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07011(3µg)+ Hela whole cell lysate treated with Pervanadate(1mg); Lane 3: Hela whole cell lysate treated with Pervanadate(20µg);
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Phospho-Cdk2 (Y15) Recombinant Antibody, Cat#RAC07011 Western Blot; Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not); All lanes:Phospho-CDK2 antibody at 0.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34 KDa; Observed band size: 34 KDa; Immunocytochemistry analysis diluted at 1:80 and staining in Hela cells(treated with Pervanadate) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating Phospho-CDK2 in Hela whole cell lysate treated with Pervanadate; Lane 1: Rabbit control IgG(1µg)instead in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07011(3µg)+ Hela whole cell lysate treated with Pervanadate(1mg); Lane 3: Hela whole cell lysate treated with Pervanadate(20µg);

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Phospho-Cdk2 (Y15) Recombinant Antibody

BiomatikSKU: RAC07011-100ul
Size: 100ul
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Western Blot; Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not); All lanes:Phospho-CDK2 antibody at 0.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34 KDa; Observed band size: 34 KDa;
Immunocytochemistry analysis diluted at 1:80 and staining in Hela cells(treated with Pervanadate) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-CDK2 in Hela whole cell lysate treated with Pervanadate; Lane 1: Rabbit control IgG(1µg)instead in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07011(3µg)+ Hela whole cell lysate treated with Pervanadate(1mg); Lane 3: Hela whole cell lysate treated with Pervanadate(20µg);

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