Western Blot; Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate; All lanes: ALIX antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 97, 97, 31 kDa; Observed band size: 97 kDa
Overlay histogram showing Jurkat cells stained with RAC07141 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.