Pak2 Recombinant Antibody, Cat#RAC07577
Western Blot; Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue; All lanes: PAK2 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 59 kDa; Observed band size: 59 kDa
Overlay histogram showing HepG2 cells stained with RAC07577 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitating PAK2 in Raji whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07577 in Raji whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07577(2ug)+ Raji whole cell lysate(500ug); Lane 3: Raji whole cell lysate (10ug)
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Pak2 Recombinant Antibody, Cat#RAC07577 Western Blot; Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue; All lanes: PAK2 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 59 kDa; Observed band size: 59 kDa Overlay histogram showing HepG2 cells stained with RAC07577 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed. Immunoprecipitating PAK2 in Raji whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07577 in Raji whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07577(2ug)+ Raji whole cell lysate(500ug); Lane 3: Raji whole cell lysate (10ug)

Pak2 Recombinant Antibody

BiomatikSKU: RAC07577-100ul
Size: 100ul
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Western Blot; Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue; All lanes: PAK2 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 59 kDa; Observed band size: 59 kDa
Overlay histogram showing HepG2 cells stained with RAC07577 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106 cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitating PAK2 in Raji whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07577 in Raji whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07577(2ug)+ Raji whole cell lysate(500ug); Lane 3: Raji whole cell lysate (10ug)

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We were very pleased with Biomatik’s performance on a custom recombinant protein project earlier this summer. Communication throughout the project was excellent, technical questions were addressed promptly, and the supporting analytical and QC data were clear and comprehensive. The recombinant protein was produced to the requested specifications and delivered on schedule. Based on this experience, we have chosen Biomatik for an additional custom protein production project and would be pleased to work with them again.

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A reliable antibody and peptide synthesis company. We’ve been using Biomtik for polyconal antibodies in our research. Turnaround times are consistent, and communication is always clear and professional.

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Biomatik provided excellent support to our Biometrology team at NPL, with timely and reliable protein production services. The quality of the material delivered was to the expected standards, and the communication throughout the project was smooth and efficient.

Andrei Baluna

We got help from Biomatik for our studies, and the experience was excellent. The customer support team was responsive and knowledgeable, providing detailed documentation and timely updates throughout the process. We appreciate the reliable quality and professional service, and we look forward to working with Biomatik again for further.

Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

Eduardo Peña

We have ordered peptides from Biomatik several times. Their quality is excellent and they work with you every step of the way to ensure a quality product.

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We have purchased peptides from Biomatik for a few years now. Their ordering process was smooth, customer service was responsive and helpful, and the peptides arrived on time and of great quality.

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Great service. I'm a returning customer. The product quality and timeline are both outstanding.

Yue Home Liu

We have used Biomatik to generate two different antibodies and in both cases they worked extremely well in all the assays we tested, including ELISA, WB, and IFA. I would recommend this company.

Sarah Wootton

The peptide product is of exceptional quality and is highly recommended for its efficacy.

Haoyang Xin

I have been using Biomatik's modified peptides for a while and they deliver high quality peptides and a very good technical support

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I have bought peptides from Biomatic several times and the quality is the best!!!

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