Western Blot, Positive WB detected in: HEK293 whole cell lysate (30ug), LO2 whole cell lysate (30ug), A431 whole cell lysate (30ug), PC-3 whole cell lysate (30ug), Hela whole cell lysate (30ug), JURKAT whole cell lysate (30ug), A549 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Mouse heart tissue lysate (30ug), All lanes: NDUFB8 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 22 kDa, Observed band size: 19 kDa, Exposure time:20s
IHC image of RAC08282 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC08282 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.