NAT10 Recombinant Monoclonal Antibody, Cat#RAC08192
Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate (20ug), A549 whole cell lysate (20ug), Hela whole cell lysate (20ug), NIH/3T3 whole cell lysate (20ug), HEK293 whole cell lysate (20ug), PC-3 whole cell lysate (20ug), COLO205 whole cell lysate (20ug),Jurkat whole cell lysate (20ug),HepG2 whole cell lysate (20ug), All lanes: NAT10 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 116 kDa, Observed band size: 130 kDa, Exposure time:20s
Overlay Peak curve showing jurkat cells stained with RAC08192 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NAT10 Recombinant Monoclonal Antibody, Cat#RAC08192 Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate (20ug), A549 whole cell lysate (20ug), Hela whole cell lysate (20ug), NIH/3T3 whole cell lysate (20ug), HEK293 whole cell lysate (20ug), PC-3 whole cell lysate (20ug), COLO205 whole cell lysate (20ug),Jurkat whole cell lysate (20ug),HepG2 whole cell lysate (20ug), All lanes: NAT10 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 116 kDa, Observed band size: 130 kDa, Exposure time:20s Overlay Peak curve showing jurkat cells stained with RAC08192 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

NAT10 Recombinant Monoclonal Antibody

BiomatikSKU: RAC08192-100ul
Size: 100ul
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Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate (20ug), A549 whole cell lysate (20ug), Hela whole cell lysate (20ug), NIH/3T3 whole cell lysate (20ug), HEK293 whole cell lysate (20ug), PC-3 whole cell lysate (20ug), COLO205 whole cell lysate (20ug),Jurkat whole cell lysate (20ug),HepG2 whole cell lysate (20ug), All lanes: NAT10 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 116 kDa, Observed band size: 130 kDa, Exposure time:20s
Overlay Peak curve showing jurkat cells stained with RAC08192 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

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