Western Blot; Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli ; SARS-CoV-2 nucleocapsid antibody at 1:1000 (RAC07048); Secondary; Peroxidase-Affinipure Goat Anti-Human IgG Fc? Fragment Specific at 1/20000 dilution; Predicted band size: 48 kDa; Observed band size: 55 kDa;
The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N; Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 µg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml.
In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (RAC07048) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.
ELISA: Immobilize various types of SARS proteins at concentration of 2µg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100µg/ml, 10µg/ml and 1µg/ml. It shows the SARS-CoV-2-N Antibody (RAC07048) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.