Western Blot, Positive WB detected in: K562 whole cell lysate (20ug), PC-3 whole cell lysate (20ug), HepG2 whole cell lysate (20ug), HeLa whole cell lysate (20ug), Jurkat whole cell lysate (20ug), A549 whole cell lysate (20ug), HEK293 whole cell lysate (20ug), All lanes: MYC antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 51 kDa, Observed band size: 56 kDa, Exposure time:30s
Overlay Peak curve showing K562 cells stained with RAC08149 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.