Western Blot, Positive WB detected in: HeLa whole cell lysate (30ug), HepG2 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), A549 whole cell lysate, PC-3 whole cell lysate (30ug), K562 whole cell lysate (30ug), All lanes: MT-CO2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 26 kDa, Observed band size: 21 kDa, Exposure time: 1min
IHC image of RAC08348 diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.