MSI2 Recombinant Monoclonal Antibody, Cat#RAC08617
Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate (30ug), U-251MG whole cell lysate (30ug), HT-29 whole cell lysate (30ug), Hela whole cell lysate (30ug), K562 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes: MSI2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 35 kDa, Observed band size: 36 kDa, Exposure time:2min40s
Overlay Peak curve showing Hela cells stained with RAC08617 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.
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MSI2 Recombinant Monoclonal Antibody, Cat#RAC08617 Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate (30ug), U-251MG whole cell lysate (30ug), HT-29 whole cell lysate (30ug), Hela whole cell lysate (30ug), K562 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes: MSI2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 35 kDa, Observed band size: 36 kDa, Exposure time:2min40s Overlay Peak curve showing Hela cells stained with RAC08617 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

MSI2 Recombinant Monoclonal Antibody

BiomatikSKU: RAC08617-100ul
Size: 100ul
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Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate (30ug), U-251MG whole cell lysate (30ug), HT-29 whole cell lysate (30ug), Hela whole cell lysate (30ug), K562 whole cell lysate (30ug), Mouse brain tissue lysate (30ug), Rat brain tissue lysate (30ug), All lanes: MSI2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 35 kDa, Observed band size: 36 kDa, Exposure time:2min40s
Overlay Peak curve showing Hela cells stained with RAC08617 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.

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