MRPL28 Recombinant Monoclonal Antibody, Cat#RAC08969
Western Blot,, Positive WB detected in:HepG2 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), HEK293T whole cell lysate (30ug), Hela whole cell lysate (30ug), Jurkat whole cell lysate (30ug), MCF7 whole cell lysate (30ug), Mouse kidney tissue lysate (30ug), Rat kidney tissue lysate (30ug), All lanes: CtBP2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 49 kDa, Observed band size: 49 kDa, Exposure time:10s
Overlay Peak curve showing Hela cells stained with RAC08969 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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MRPL28 Recombinant Monoclonal Antibody, Cat#RAC08969 Western Blot,, Positive WB detected in:HepG2 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), HEK293T whole cell lysate (30ug), Hela whole cell lysate (30ug), Jurkat whole cell lysate (30ug), MCF7 whole cell lysate (30ug), Mouse kidney tissue lysate (30ug), Rat kidney tissue lysate (30ug), All lanes: CtBP2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 49 kDa, Observed band size: 49 kDa, Exposure time:10s Overlay Peak curve showing Hela cells stained with RAC08969 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.

MRPL28 Recombinant Monoclonal Antibody

BiomatikSKU: RAC08969-100ul
Size: 100ul
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Western Blot,, Positive WB detected in:HepG2 whole cell lysate (30ug), COLO205 whole cell lysate (30ug), HEK293T whole cell lysate (30ug), Hela whole cell lysate (30ug), Jurkat whole cell lysate (30ug), MCF7 whole cell lysate (30ug), Mouse kidney tissue lysate (30ug), Rat kidney tissue lysate (30ug), All lanes: CtBP2 antibody at 1:1000, Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution, Predicted band size: 49 kDa, Observed band size: 49 kDa, Exposure time:10s
Overlay Peak curve showing Hela cells stained with RAC08969 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10_6cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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Keyao Yu

I work on molecular farming, producing recombinant protein in plant suspention cells. I'm completely satisfied after almost 5 years ordering antibidies at Biomatik. Perfect reproducibility batch after batch and I hope to continiue like this!

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