Western Blot, Positive WB detected in: THP-1 whole cell lysate (30ug), HeLa whole cell lysate (30ug), All lanes: MPL antibody at 1:1000, Secondary, Goat polyclonal to human IgG at 1/40000 dilution, Predicted band size: 71 kDa, Observed band size: 100 kDa, Exposure time: 3min
Overlay Peak curve showing HL-60 cells stained with RAC08801 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10_6cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10_6cells) used under the same conditions. Acquisition of >10,000 events was performed.