Mono-Methyl-Histone H3.1 (R2) Recombinant Antibody, Cat#RAC07089
Western Blot; Positive WB detected in Hela whole cell lysate epG2 whole cell lysate H-SY5Y whole cell lysate; All lanes Mono-methyl-Histone H3.1(R2)antibody at 0.95ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 15 KDa; Observed band size: 15 KDa
Immunocytochemistry analysis of RAC07089 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07089 at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Mono-Methyl-Histone H3.1 (R2) Recombinant Antibody, Cat#RAC07089 Western Blot; Positive WB detected in Hela whole cell lysate epG2 whole cell lysate H-SY5Y whole cell lysate; All lanes Mono-methyl-Histone H3.1(R2)antibody at 0.95ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 15 KDa; Observed band size: 15 KDa Immunocytochemistry analysis of RAC07089 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with RAC07089 at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Mono-Methyl-Histone H3.1 (R2) Recombinant Antibody

BiomatikSKU: RAC07089-100ul
Size: 100ul
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Western Blot; Positive WB detected in Hela whole cell lysate epG2 whole cell lysate H-SY5Y whole cell lysate; All lanes Mono-methyl-Histone H3.1(R2)antibody at 0.95ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 15 KDa; Observed band size: 15 KDa
Immunocytochemistry analysis of RAC07089 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07089 at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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